Email      Password  
          Sign in  |   e-buy  |   Training
 
INTERFINE CHEMICALS
ANALYTICAL SCIENCES
BIOCHROMATOGRAPHY
LIFE SCIENCES
SUPPLIERS
BIOCHROMATOGRAPHY > Affinity > Bio-Works IMAC
Logo_Bio_Works_Interchim_0416 Bio-Works, a Sweden company, designs, develops and manufactures a broad product range in Uppsala.
For uses within areas of Life Sciences, Food & Beverage.

WorkBeads™ 40 Ni

High throughput agarose media for capture of His-tagged proteins.

  • High dynamic capacity
  • High purity
  • Excellent flow properties

Applications
WorkBeads 40 Ni is developed for capture of His-tagged proteins. Typical binding and wash buffer is 20 mM sodium phosphate pH 7.4 with 0.5 M NaCl. In order to reduce unspecific binding 20-40 mM imidazole is added to the buffer and the amount is protein dependent and should be tried out. Elution is carried out by further addition of 300-500 mM imidazole and is also protein dependent.

Media characteristics WorkBeads 40 Ni
Agarose content
7%
Metal Ion capacity (μmol Ni2+/ml) 50-60
Average particle Size (μm) 45
Protein capacity (mg/ml) >60
Max flow rate (cm/h)
(20cm Bed Height, 5 Bar)
600
pH stability 45

 

WorkBeads™ 40 IDAhigh
WorkBeads™ 40 IDAlow
WorkBeads™ 40 TRENhigh
WorkBeads™ 40 TRENlow

Affinity chromatography media for laboratory and process scale users.

  • Faster to results; simple coupling procedure
  • Stable at ambient temperature, aqueous coupling solution and neutral pH
  • Suitable for coupling ligands containing Sulphydryl-, Amino- or Hydroxylgroups

Applications
Not all proteins behave the same and this is the reason for having four different varieties of IMAC chemistries. Start with IDA high as this will have the best capacity and works well for most of the proteins. If proteins are difficult to desorb or are eluted with less activity it is an indication of too strong binding, in this case try IDA low. If this will not give you the result try TREN high followed by TREN low. In this way you will have good probability to find the media that will fit your application.

Media characteristics WorkBeads 40 IDA WorkBeads 40 TREN
Agarose content
7% 7%
Chelating group Iminodiacetic acid (IDA) Tris(2-ethylaminoethyl) amine (TREN)
Metal Ion capacity (μmol Cu2+/ml) 10-20 IDAlow
50-60 IDAhigh
10-20 TRENlow
50-60 TRENhigh
Average particle size (μm) 45 45
Max flow rate (cm/hr)
(20cm bed height,  5 Bar)
600 600
pH stability 2-13 2-13
Search : Bio-Works IMAC
   Not   
Advanced Search : Bio-Works IMAC
 
 Sort by : Part number Alphabetical 25 50
54 products
  1  2  3  >  >>
BIO-WORKS BIO-WORKS BABYBIO A PROTEIN A AFFINITY 1 ML MINICOLUMN
#nogo
P/N : 45605104     Pack : 10 x 1 ml  
P/N : 45605104         Item : 1M1793         Pack : 10 x 1 ml  
Cat. Price :




 
 
 
BIO-WORKS BIO-WORKS BABYBIO A PROTEIN A AFFINITY 1 ML MINICOLUMN
#nogo
P/N : 45605103     Pack : 05 x 1 ml  
P/N : 45605103         Item : 1M1792         Pack : 05 x 1 ml  
Cat. Price :




 
 
 
BIO-WORKS BIO-WORKS BABYBIO A PROTEIN A AFFINITY 1 ML MINICOLUMN
#nogo
P/N : 45605102     Pack : 02 x 1 ml  
P/N : 45605102         Item : 1M1791         Pack : 02 x 1 ml  
Cat. Price :




 
 
 
BIO-WORKS BIO-WORKS BABYBIO A PROTEIN A AFFINITY 1 ML MINICOLUMN
#nogo
P/N : 45605101     Pack : 01 x 1 ml  
P/N : 45605101         Item : 1M1790         Pack : 01 x 1 ml  
Cat. Price :




 
 
 
BIO-WORKS BIO-WORKS BABYBIO A PROTEIN A AFFINITY 5 ML MINICOLUMN
#nogo
P/N : 45605105     Pack : 01 x 5 ml  
P/N : 45605105         Item : 1M1800         Pack : 01 x 5 ml  
Cat. Price :




 
 
 
BIO-WORKS BIO-WORKS BABYBIO A PROTEIN A AFFINITY 5 ML MINICOLUMN
#nogo
P/N : 45605108     Pack : 10 x 5 ml  
P/N : 45605108         Item : 1M1803         Pack : 10 x 5 ml  
Cat. Price :




 
 
 
BIO-WORKS BIO-WORKS BABYBIO A PROTEIN A AFFINITY 5 ML MINICOLUMN
#nogo
P/N : 45605107     Pack : 05 x 5 ml  
P/N : 45605107         Item : 1M1802         Pack : 05 x 5 ml  
Cat. Price :




 
 
 
BIO-WORKS BIO-WORKS BABYBIO A PROTEIN A AFFINITY 5 ML MINICOLUMN
#nogo
P/N : 45605106     Pack : 02 x 5 ml  
P/N : 45605106         Item : 1M1801         Pack : 02 x 5 ml  
Cat. Price :




 
 
 
BIO-WORKS BIO-WORKS BABYBIO DSALT DESALTING 1ML MINICOLUMN
#nogo
P/N : 45360103     Pack : 05 x 1 ml  
P/N : 45360103         Item : 1M1770         Pack : 05 x 1 ml  
Cat. Price :




 
 
 
BIO-WORKS BIO-WORKS BABYBIO DSALT DESALTING 1ML MINICOLUMN
#nogo
P/N : 45360104     Pack : 10 x 1 ml  
P/N : 45360104         Item : 1M1771         Pack : 10 x 1 ml  
Cat. Price :




 
 
 
BIO-WORKS BIO-WORKS BABYBIO DSALT DESALTING 1ML MINICOLUMN
#nogo
P/N : 45360110     Pack : 99 x 1 ml  
P/N : 45360110         Item : 1M1772         Pack : 99 x 1 ml  
Cat. Price :




 
 
 
BIO-WORKS BIO-WORKS BABYBIO DSALT DESALTING 5 ML MINICOLUMN
#nogo
P/N : 45360109     Pack : 99 x 5 ml  
P/N : 45360109         Item : 1M1784         Pack : 99 x 5 ml  
Cat. Price :




 
 
 
BIO-WORKS BIO-WORKS BABYBIO DSALT DESALTING 5 ML MINICOLUMN
#nogo
P/N : 45360105     Pack : 01 x 5 ml  
P/N : 45360105         Item : 1M1780         Pack : 01 x 5 ml  
Cat. Price :




 
 
 
BIO-WORKS BIO-WORKS BABYBIO DSALT DESALTING 5 ML MINICOLUMN
#nogo
P/N : 45360108     Pack : 10 x 5 ml  
P/N : 45360108         Item : 1M1783         Pack : 10 x 5 ml  
Cat. Price :




 
 
 
BIO-WORKS BIO-WORKS BABYBIO DSALT DESALTING 5 ML MINICOLUMN
#nogo
P/N : 45360106     Pack : 02 x 5 ml  
P/N : 45360106         Item : 1M1781         Pack : 02 x 5 ml  
Cat. Price :




 
 
 
BIO-WORKS BIO-WORKS BABYBIO DSALT DESALTING 5 ML MINICOLUMN
#nogo
P/N : 45360107     Pack : 05 x 5 ml  
P/N : 45360107         Item : 1M1782         Pack : 05 x 5 ml  
Cat. Price :




 
 
 
BIO-WORKS BIO-WORKS BABYBIO NINTA NICKEL IMAC 1 ML MINICOLUMN
#nogo
P/N : 45655110     Pack : 99 x 1 ml  
P/N : 45655110         Item : 1M1823         Pack : 99 x 1 ml  
Cat. Price :




 
 
 
BIO-WORKS BIO-WORKS BABYBIO NINTA NICKEL IMAC 1 ML MINICOLUMN
#nogo
P/N : 45655104     Pack : 10 x 1 ml  
P/N : 45655104         Item : 1M1822         Pack : 10 x 1 ml  
Cat. Price :




 
 
 
BIO-WORKS BIO-WORKS BABYBIO NINTA NICKEL IMAC 1 ML MINICOLUMN
#nogo
P/N : 45655103     Pack : 05 x 1 ml  
P/N : 45655103         Item : 1M1821         Pack : 05 x 1 ml  
Cat. Price :




 
 
 
BIO-WORKS BIO-WORKS BABYBIO NINTA NICKEL IMAC 1 ML MINICOLUMN
#nogo
P/N : 45655102     Pack : 02 x 1 ml  
P/N : 45655102         Item : 1M1820         Pack : 02 x 1 ml  
Cat. Price :




 
 
 
BIO-WORKS BIO-WORKS BABYBIO NINTA NICKEL IMAC 5 ML MINI COLUMN
#nogo
P/N : 45655105     Pack : 01 x 5 ml  
P/N : 45655105         Item : 1M1830         Pack : 01 x 5 ml  
Cat. Price :




 
 
 
BIO-WORKS BIO-WORKS BABYBIO NINTA NICKEL IMAC 5 ML MINI COLUMN
#nogo
P/N : 45655106     Pack : 02 x 5 ml  
P/N : 45655106         Item : 1M1831         Pack : 02 x 5 ml  
Cat. Price :




 
 
 
BIO-WORKS BIO-WORKS BABYBIO NINTA NICKEL IMAC 5 ML MINI COLUMN
#nogo
P/N : 45655107     Pack : 05 x 5 ml  
P/N : 45655107         Item : 1M1832         Pack : 05 x 5 ml  
Cat. Price :




 
 
 
BIO-WORKS BIO-WORKS BABYBIO NINTA NICKEL IMAC 5 ML MINI COLUMN
#nogo
P/N : 45655108     Pack : 10 x 5 ml  
P/N : 45655108         Item : 1M1833         Pack : 10 x 5 ml  
Cat. Price :




 
 
 
BIO-WORKS BIO-WORKS BABYBIO NINTA NICKEL IMAC 5 ML MINI COLUMN
#nogo
P/N : 45655109     Pack : 99 x 5 ml  
P/N : 45655109         Item : 1M1834         Pack : 99 x 5 ml  
Cat. Price :




 
 
 
  1  2  3  >  >>
  Products highlight


Site map | Contact us | About us | Terms & Conditions | Legal notice
   
                Interchim | 211 Bis avenue J.F. Kennedy - BP 1140 | 03100 Montluçon FRANCE |
              Phone: +33 4 70 03 88 55 | Fax: +33 4 70 03 82 60 | interchim@interchim.com