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BIOCHROMATOGRAPHY > Affinity > Bio-Works IMAC
Logo_Bio_Works_Interchim_0416 Bio-Works, a Sweden company, designs, develops and manufactures a broad product range in Uppsala.
For uses within areas of Life Sciences, Food & Beverage.

WorkBeads™ 40 Ni

High throughput agarose media for capture of His-tagged proteins.

  • High dynamic capacity
  • High purity
  • Excellent flow properties

Applications
WorkBeads 40 Ni is developed for capture of His-tagged proteins. Typical binding and wash buffer is 20 mM sodium phosphate pH 7.4 with 0.5 M NaCl. In order to reduce unspecific binding 20-40 mM imidazole is added to the buffer and the amount is protein dependent and should be tried out. Elution is carried out by further addition of 300-500 mM imidazole and is also protein dependent.

Media characteristics WorkBeads 40 Ni
Agarose content
7%
Metal Ion capacity (μmol Ni2+/ml) 50-60
Average particle Size (μm) 45
Protein capacity (mg/ml) >60
Max flow rate (cm/h)
(20cm Bed Height, 5 Bar)
600
pH stability 45

 

WorkBeads™ 40 IDAhigh
WorkBeads™ 40 IDAlow
WorkBeads™ 40 TRENhigh
WorkBeads™ 40 TRENlow

Affinity chromatography media for laboratory and process scale users.

  • Faster to results; simple coupling procedure
  • Stable at ambient temperature, aqueous coupling solution and neutral pH
  • Suitable for coupling ligands containing Sulphydryl-, Amino- or Hydroxylgroups

Applications
Not all proteins behave the same and this is the reason for having four different varieties of IMAC chemistries. Start with IDA high as this will have the best capacity and works well for most of the proteins. If proteins are difficult to desorb or are eluted with less activity it is an indication of too strong binding, in this case try IDA low. If this will not give you the result try TREN high followed by TREN low. In this way you will have good probability to find the media that will fit your application.

Media characteristics WorkBeads 40 IDA WorkBeads 40 TREN
Agarose content
7% 7%
Chelating group Iminodiacetic acid (IDA) Tris(2-ethylaminoethyl) amine (TREN)
Metal Ion capacity (μmol Cu2+/ml) 10-20 IDAlow
50-60 IDAhigh
10-20 TRENlow
50-60 TRENhigh
Average particle size (μm) 45 45
Max flow rate (cm/hr)
(20cm bed height,  5 Bar)
600 600
pH stability 2-13 2-13
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BIO-WORKS BIO-WORKS BABYBIO ACT AFFINITY 5ML MINICOLUMN
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P/N : 45400005     Pack : 1 x 5 ml  
P/N : 45400005         Item : A2ZAR0         Pack : 1 x 5 ml  
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BIO-WORKS BIO-WORKS BABYBIO ACT AFFINITY 1ML MINICOLUMN
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P/N : 45400001     Pack : 1 x 1 ml  
P/N : 45400001         Item : A2ZAQ0         Pack : 1 x 1 ml  
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BIO-WORKS BIO-WORKS BABYBIO ACT AFFINITY 1ML MINICOLUMN
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P/N : 45400002     Pack : 2 x 1 ml  
P/N : 45400002         Item : A2ZAQ1         Pack : 2 x 1 ml  
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BIO-WORKS BIO-WORKS BABYBIO ACT AFFINITY 1ML MINICOLUMN
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P/N : 45400003     Pack : 5 x 1 ml  
P/N : 45400003         Item : A2ZAQ2         Pack : 5 x 1 ml  
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BIO-WORKS BIO-WORKS BABYBIO ACT AFFINITY 1ML MINICOLUMN
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P/N : 45400004     Pack : 10 x 1 ml  
P/N : 45400004         Item : A2ZAQ3         Pack : 10 x 1 ml  
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BIO-WORKS BIO-WORKS BABYBIO ACT AFFINITY 5ML MINICOLUMN
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P/N : 45400008     Pack : 10 x 5 ml  
P/N : 45400008         Item : A2ZAS2         Pack : 10 x 5 ml  
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BIO-WORKS BIO-WORKS BABYBIO ACT AFFINITY 5ML MINICOLUMN
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P/N : 45400006     Pack : 2 x 5 ml  
P/N : 45400006         Item : A2ZAS0         Pack : 2 x 5 ml  
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BIO-WORKS BIO-WORKS BABYBIO ACT AFFINITY 5ML MINICOLUMN
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P/N : 45400007     Pack : 5 x 5 ml  
P/N : 45400007         Item : A2ZAS1         Pack : 5 x 5 ml  
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BIO-WORKS BIO-WORKS BABYBIO A PROTEIN A AFFINITY 1ML MINICOLUMN
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P/N : 45605101     Pack : 1 x 1 ml  
P/N : 45605101         Item : 1M1790         Pack : 1 x 1 ml  
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BIO-WORKS BIO-WORKS BABYBIO A PROTEIN A AFFINITY 1ML MINICOLUMN
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P/N : 45605104     Pack : 10 x 1 ml  
P/N : 45605104         Item : 1M1793         Pack : 10 x 1 ml  
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BIO-WORKS BIO-WORKS BABYBIO A PROTEIN A AFFINITY 1ML MINICOLUMN
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P/N : 45605103     Pack : 5 x 1 ml  
P/N : 45605103         Item : 1M1792         Pack : 5 x 1 ml  
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BIO-WORKS BIO-WORKS BABYBIO A PROTEIN A AFFINITY 1ML MINICOLUMN
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P/N : 45605102     Pack : 2 x 1 ml  
P/N : 45605102         Item : 1M1791         Pack : 2 x 1 ml  
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BIO-WORKS BIO-WORKS BABYBIO A PROTEIN A AFFINITY 5ML MINICOLUMN
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P/N : 45605108     Pack : 10 x 5 ml  
P/N : 45605108         Item : 1M1803         Pack : 10 x 5 ml  
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BIO-WORKS BIO-WORKS BABYBIO A PROTEIN A AFFINITY 5ML MINICOLUMN
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P/N : 45605107     Pack : 5 x 5 ml  
P/N : 45605107         Item : 1M1802         Pack : 5 x 5 ml  
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BIO-WORKS BIO-WORKS BABYBIO A PROTEIN A AFFINITY 5ML MINICOLUMN
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P/N : 45605106     Pack : 2 x 5 ml  
P/N : 45605106         Item : 1M1801         Pack : 2 x 5 ml  
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BIO-WORKS BIO-WORKS BABYBIO A PROTEIN A AFFINITY 5ML MINICOLUMN
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P/N : 45605105     Pack : 1 x 5 ml  
P/N : 45605105         Item : 1M1800         Pack : 1 x 5 ml  
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BIO-WORKS WORKBEADS 40 PROTEIN A 40UM
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P/N : 40605005     Pack : 01 x 1 L  
P/N : 40605005         Item : A2ZAP4         Pack : 01 x 1 L  
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BIO-WORKS WORKBEADS 40 PROTEIN A 40UM
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P/N : 40605004     Pack : 01 x 100 ml  
P/N : 40605004         Item : A2ZAP3         Pack : 01 x 100 ml  
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BIO-WORKS WORKBEADS 40 PROTEIN A 40UM
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P/N : 40605003     Pack : 01 x 10 ml  
P/N : 40605003         Item : A2ZAP2         Pack : 01 x 10 ml  
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BIO-WORKS WORKBEADS 40 PROTEIN A 40UM
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P/N : 40605002     Pack : 01 x 5 ml  
P/N : 40605002         Item : A2ZAP1         Pack : 01 x 5 ml  
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BIO-WORKS WORKBEADS 40 PROTEIN A 40UM
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P/N : 40605001     Pack : 01 x 1.5 ml  
P/N : 40605001         Item : A2ZAP0         Pack : 01 x 1.5 ml  
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BIO-WORKS WORKBEADS 40 PROTEIN A 40U
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P/N : 40605001     Pack : 01 x 1.5 ml  
P/N : 40605001         Item : 7A2040         Pack : 01 x 1.5 ml  
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BIO-WORKS WORKBEADS 40 PROTEIN A 40U
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P/N : 40605002     Pack : 01 x 5 ml  
P/N : 40605002         Item : 7A2041         Pack : 01 x 5 ml  
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BIO-WORKS WORKBEADS 40 PROTEIN A 40U
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P/N : 40605003     Pack : 01 x 10 ml  
P/N : 40605003         Item : 7A2042         Pack : 01 x 10 ml  
Cat. Price :




 
 
 
BIO-WORKS WORKBEADS 40 PROTEIN A 40U
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P/N : 40605004     Pack : 01 x 100 ml  
P/N : 40605004         Item : 7A2043         Pack : 01 x 100 ml  
Cat. Price :




 
 
 
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