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LIFE SCIENCES > Immunodetection Tools > COVID-19 RESEARCH > RT-qPCR and RNA extraction kits

Covid-19: RT-qPCR and RNA extraction kits

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We offer a range of RT-qPCR kits for testing the new coronavirus strain COVID-19. Our molecular kits are specific in obtaining accurate results, specially in avoiding false-negative outcomes.  
   
   

Total RNA Purification Kit

   
For rapid purification of total RNA - including microRNA - without phenol.
  • Extract high quality & purity total RNA including miRNA
  • No phenol step required
  • Bind & elute all RNA irrespective of size or GC content, without bias
  • Efficiently extract small RNA irrespective of GC content
  • Very sensitive & linear down to a few cells without the need for carrier RNA
  • Convenient & fast spin column format
  • Isolate from a wide variety of specimens
  • Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing.
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Ref.   Description   Packing
17200   Total RNA Purification Kit   50 preps
37500   Total RNA Purification Kit   100 preps
17250   Total RNA Purification Kit   250 preps
17270   Total RNA Purification Kit   500 preps
24350   Total RNA Purification 96-Well Kit (Deep Well Format, height 3.8 cm)   2 plates
24380   Total RNA Purification 96-Well Kit (Deep Well Format, height 3.8 cm)   6 plates
         
         

2019-nCoV TaqMan RT-PCR Kit

   
Norgen’s 2019-nCoV TaqMan RT-PCR Kit is designed for the detection of SARS-CoV-2 specific RNA in a real-time RT-PCR based on the use of TaqMan technology. This kit is designed for research use only and not for use in diagnostic procedures. The detection of SARS-CoV-2 specific RNA is based on TaqMan one-step RT-PCR providing a simple, reliable and rapid result for the detection of SARS-CoV-2 infection. Norgen’s 2019-nCoV TaqMan RT-PCR Kit includes a PCR control to monitor for PCR inhibition, and to validate the quality of the sample and the detection result.   RT-qPCR_Covid-19_coronavirus_research_Interchim01_0420
The 2019-nCoV TaqMan RT-PCR Kit comprises Master Mix for the target and PCR control detection, 4 target Primer & Probe Mixes, as well as a positive control and a negative control (nuclease-free water) to confirm the integrity of the kit reagen.
     
     

Detection kit for SARS-CoV-2, which causes COVID-19 (2019-nCoV):

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Ready to use format containing everything required for detection Available in 1 format for analysis  
     
Ref.   Description   Packing
TM67100   2019-nCoV TaqMan RT-PCR Kit   50 reactions
TM67120   2019-nCoV TaqMan RT-PCR Kit   500 reactions
         
         

PCR Detection Solutions for Coronavirus

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For rapid purification of total RNA - including microRNA - without phenol.
  • COVID-19 detection based on the assays and protocols developed by the CDC
  • Primer & Probe Mixes contain all 3 CDC developed assays in individual tubes
  • All assays are premixed to the working concentrations recommended by the CDC
  • Positive Control contains 2 nCoV nucleocapsid target gene RNA (N1,N2) and RNase P (internal control) which are compatible with the CDC primer/probe set
  • Convenient ready-to-use 2X One-Step RT-PCR Master Mix
  • Manufactured under ISO 13485:2016 for research use only
     
Ref.   Description   Packing
TM67101   2019-nCoV Primer & Probe Mixes   50 reactions (per tube)
PC67102   2019-nCoV RT-PCR Positive Control   50 µL
TM67130   2019-nCoV Primer & Probe Mixes   500 reactions (per tube)
PC67120   2019-nCoV Positive Control   500 µL
28113   2X One-Step RT-PCR Master Mix   100 reactions
28115   2X One-Step RT-PCR Master Mix   500 reactions
         
         

Fast Plus Evagreen qPCR Master Mix

   
Fast Plus EvaGreen® qPCR master mix is a ready-to-use hot-start mix for nucleic acid quantitation and melt curve analysis of PCR amplicons. Formulated using the environmentally safe EvaGreen® dye and fast-activating chemically-modified hotstart enzyme, Cheetah™ Taq, Biotium´s PCR master mix delivers superior performance even with the most challenging samples tested.   Logo_Biotium_Interchim_0420
     
Covid-19_coronavirus_research_Interchim013_0420   EvaGreen® PCR dye is bright and sensitive. Comparison between an EvaGreen® qPCR Master Mix from Biotium and two fast SYBR® Green master mixes from two leading companies (company A and company Q) under similar condition. The inset is an enlarged view of the area near the baseline for better viewing the curve patterns of the weaker signals of the two SYBR®-based master mixes. Amplicon: ATPG fragment of human genomic DNA; instrument: ABI 7900 Fast.
     
The master mix is offered in three formats containing different amounts of ROX reference dye for use with different PCR instruments (without ROX, low ROX, and high ROX).
     
Ref.   Description   Packing
#31014-T   FAST PLUS EVAGREEN qPCR MASTER MIX - Low ROX   Trial size (1 mL)
#31014   FAST PLUS EVAGREEN qPCR MASTER MIX - Low ROX   2 x 1 mL
#31014-1   FAST PLUS EVAGREEN qPCR MASTER MIX - Low ROX   5 x 1 mL
#31014-2   FAST PLUS EVAGREEN qPCR MASTER MIX - Low ROX   50 x 1 mL
         
         

ABScript II One Step RT-qPCR Probe Kit (RK20407)

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ABScript II One Step RT-qPCR Probe Kit a ready-to-use kit allowing reverse transcription and subsequent probe-based qPCR in a single tube. It contains all components for RT-qPCR except primers, probes and RNA templates.

The one-step format significantly improves sensitivity and effectively prevent contamination.
The ABScript II Reverse Transcriptase in the kit provides reliable reverse transcription to a wide range of RNA template amount. After reverse transcription, the Hot-start version of Taq polymerase is activated at 95°C and the ABScript II Reverse Transcriptase is inactivated simultaneously. In the sequential PCR reaction, the 5 ´-3 ´exonuclease activity of Taq polymerase cleaves the hybridized probe, separating the reporter from the quencher and releasing fluorescent signal. The ABScript II One Step RT-qPCR Probe Kit is an ideal product for high-speed analyses of low input RNA sample.

Features :
    • From RNA sample to qPCR data only need one step;
    • The core reverse transcripase in this kit is ABclonal´s ABScript II, which could work in various situations. It has advantage of sensitivity and efficiency;
    • Suitable for multiplex PCR;
    • Could be applied in research, clinical /medical, industrial probe-based RT-qPCR reactions;
    • Compatible for all machine types, choosing the ROX types as you wish 
     

Result Using synthesed sequence from 2019-nCov
 

 
RT-qPCR_Covid-19_coronavirus_research_Interchim02_0420 Using the Pseudovirus, synthesed sequence from 2019-nCov, as the template, diluted into 105, 104, 103, 102 and 10 copies. Targets are ORF 1ab gene and N gene (Probes are recommended by CCDC).
The target could be well detected by ABclonal´s kit.
     
Ref.   Description   Packing
RK20407   ABScript II One Step RT-qPCR Probe Kit   50 reactions
RK20407   ABScript II One Step RT-qPCR Probe Kit   100 reactions


 
       

For more information, please see our Covid-19 related article (in french).

In progress. Due to the current situation, this page may be subject to frequent updates.
For any request please contact-us.

 

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ABCLONAL BIOTECH ABSCRIPT II ONE STEP RT-QPCR PROBE KIT
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P/N : RK20407     Pack : 01 x 20 TS  
P/N : RK20407         Item : B8D8S0         Pack : 01 x 20 TS  
Cat. Price :




 
 
 
ABCLONAL BIOTECH ABSCRIPT II ONE STEP RT-QPCR PROBE KIT
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P/N : RK20407     Pack : 01 x 100 TS  
P/N : RK20407         Item : B8D8S1         Pack : 01 x 100 TS  
Cat. Price :




 
 
 
ABM GenomeCoV19 Detection Kit, CE-marked (for IVD) naso/oropharyngeal COVID-19 SARS-CoV-2 by RT-qPCR
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P/N : G628     Pack : 01 x 100 TS  
P/N : G628         Item : B8DRW0         Pack : 01 x 100 TS  
Cat. Price :




 
 
BIOMAXIMA SARS-CoV-2 Real Time PCR LAB-KIT (CE mark, IVD), 8-well strips
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P/N : PCR5008     Pack : 01 x 96 TS  
P/N : PCR5008         Item : B8DHQ0         Pack : 01 x 96 TS  
Cat. Price :




 
 
BIOMAXIMA SARS-CoV-2 Real Time PCR LAB-KIT (CE mark, IVD), 96-well plate
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P/N : PCR5096     Pack : 01 x 96 TS  
P/N : PCR5096         Item : B8DHR0         Pack : 01 x 96 TS  
Cat. Price :




 
 
BIORBYT VIRUS NUCLEIC ACID ISOLATION KIT
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P/N : ORB180514     Pack : 1 x 100 TS  
P/N : ORB180514         Item : WKS200         Pack : 1 x 100 TS  
Cat. Price :




 
 
FAVORGEN BIOTECH TISSUE TOTAL RNA MINI KIT
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P/N : FATRK001-2     Pack : 1 x 300 TS  
P/N : FATRK001-2         Item : EMV752         Pack : 1 x 300 TS  
Cat. Price :




 
 
FAVORGEN BIOTECH TISSUE TOTAL RNA MINI KIT
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P/N : FATRK001     Pack : 1 x 50 TS  
P/N : FATRK001         Item : EMV750         Pack : 1 x 50 TS  
Cat. Price :




 
 
FAVORGEN BIOTECH TISSUE TOTAL RNA MINI KIT
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P/N : FATRK001-1     Pack : 1 x 100 TS  
P/N : FATRK001-1         Item : EMV751         Pack : 1 x 100 TS  
Cat. Price :




 
 
FAVORGEN BIOTECH VIRAL NUCLEIC ACID EXTRACTION KIT I, for low viral load specimen using carrier RNA
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P/N : FAVNK001     Pack : 1 x 50 TS  
P/N : FAVNK001         Item : EMV800         Pack : 1 x 50 TS  
Cat. Price :




 
 
FAVORGEN BIOTECH VIRAL NUCLEIC ACID EXTRACTION KIT I, for low viral load specimen using carrier RNA
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P/N : FAVNK001-1     Pack : 1 x 100 TS  
P/N : FAVNK001-1         Item : EMV801         Pack : 1 x 100 TS  
Cat. Price :




 
 
FAVORGEN BIOTECH VIRAL NUCLEIC ACID EXTRACTION KIT I, for low viral load specimen using carrier RNA
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P/N : FAVNK001-2     Pack : 1 x 300 TS  
P/N : FAVNK001-2         Item : EMV802         Pack : 1 x 300 TS  
Cat. Price :




 
 
FAVORGEN BIOTECH VIRAL NUCLEIC ACID EXTRACTION KIT II, For isolation from cell-free fluid (serum, plasma, body fluid, cell supern
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P/N : FAVNK002     Pack : 1 x 50 TS  
P/N : FAVNK002         Item : EMV830         Pack : 1 x 50 TS  
Cat. Price :




 
 
FAVORGEN BIOTECH VIRAL NUCLEIC ACID EXTRACTION KIT II, For isolation from cell-free fluid (serum, plasma, body fluid, cell supern
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P/N : FAVNK002-1     Pack : 1 x 100 TS  
P/N : FAVNK002-1         Item : EMV831         Pack : 1 x 100 TS  
Cat. Price :




 
 
FAVORGEN BIOTECH VIRAL NUCLEIC ACID EXTRACTION KIT II, For isolation from cell-free fluid (serum, plasma, body fluid, cell supern
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P/N : FAVNK002-2     Pack : 1 x 300 TS  
P/N : FAVNK002-2         Item : EMV832         Pack : 1 x 300 TS  
Cat. Price :




 
 
GENEDIREX COVID-19 RT-qPCR Detection Kit
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P/N : QP019-0100     Pack : 01 x 100 TS  
P/N : QP019-0100         Item : B8DGN0         Pack : 01 x 100 TS  
Cat. Price :




 
 
GENEDIREX VIRUS NUCLEIC ACID ISOLATION KIT
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P/N : PDC03-0100     Pack : 1 x 100 TS  
P/N : PDC03-0100         Item : WKS202         Pack : 1 x 100 TS  
Cat. Price :




 
 
GENEDIREX VIRUS NUCLEIC ACID ISOLATION KIT
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P/N : PDC03-0100S     Pack : 1 x 4 TS  
P/N : PDC03-0100S         Item : WKS204         Pack : 1 x 4 TS  
Cat. Price :




 
 
INTRON BIOTECHNOLOGY LILIF COVID-19 REAL-TIME RT-PCR KIT (IVD)
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P/N : IPH21505     Pack : 01 x 48 TS  
P/N : IPH21505         Item : B8CJY0         Pack : 01 x 48 TS  
Cat. Price :




 
 
NORGEN BIOTEK 2019-nCoV TaqMan RT-PCR Kit, Detection kit for SARS-CoV-2, which causes COVID-19, CE-IVD marked
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P/N : TM67120     Pack : 01 x 500 TS  
P/N : TM67120         Item : B8D9IB         Pack : 01 x 500 TS  
Cat. Price :




 
 
 
NORGEN BIOTEK 2019-nCoV TaqMan RT-PCR Kit, Detection kit for SARS-CoV-2, which causes COVID-19, CE-IVD marked
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P/N : TM67100     Pack : 01 x 50 TS  
P/N : TM67100         Item : B8D9IA         Pack : 01 x 50 TS  
Cat. Price :




 
 
NORGEN BIOTEK COVID-19 TaqMan RT-PCR Kit (E/RdRP genes-Charité/Berlin protocol), CE-IVD marked
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P/N : TM67200     Pack : 01 x 500 TS  
P/N : TM67200         Item : B8J0C0         Pack : 01 x 500 TS  
Cat. Price :




 
 
NORGEN BIOTEK Saliva - Swab RNA Purification Kit Dx, CE-IVD marked
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P/N : DX69100     Pack : 01 x 50 TS  
P/N : DX69100         Item : BFW8Q0         Pack : 01 x 50 TS  
Cat. Price :




 
 
NORGEN BIOTEK Saliva/Swab RNA Purification 96-Well Kit Dx, CE-IVD marked
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P/N : DX69300     Pack : 02 x 96 TS  
P/N : DX69300         Item : BFW8V0         Pack : 02 x 96 TS  
Cat. Price :




 
 
NORGEN BIOTEK Saliva/Swab RNA Purification 96-Well Kit
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P/N : 69300     Pack : 02 x 96 TS  
P/N : 69300         Item : BFW8W0         Pack : 02 x 96 TS  
Cat. Price :




 
 
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