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BIOCHROMATOGRAPHY > Affinity > Uptisphere

Wide Pore Silica

Uptisphere® Protein A HPLC columns combine the pressure benefits associated with HPLC and a silica based stationary phase with the affinity binding characteristics of Protein A. These columns can generally run on HPLC apparatus up to flow rates of 720cm /hr compared to classical Protein A agarose based affinity columns that work up to flow rates of 50 - to 60cm /hr.

Uptisphere® Protein A bonded silica is generally used for Immunglobulin purification
from research to process scale.

  • No issues of swelling
  • High mechanical stability
  • Thermal stability
  • Compatible with water and organic solvents
  • High capacity at high flow velocities: 45mg/ml
  • Protein recovery: 98 - 100%
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