Do the TSKgel PW/PWXL columns shed microparticles?
FAQ from TOSOH
Microparticles do not interfere with most detection methods used in HPLC. However, these particles can increase the noise level in laser light scattering detection systems. To minimize this effect using the PW/PWXL columns run the column overnight in 10-20% methanol at one-half the normal flow rate to clean any microparticles off the resin.