How is the AutoSampler injection volume determined?
The injection volume depends both on the volume of the tube in the sample rack and on the injection loop on the injection valve (the sample volume is limited by the loop capacity).
If you wish to inject large volumes, you can achieve this by modulating the syringe volume (10 ml syringe by default - optional 25 ml syringe).
You can also perform several filling cycles with the syringe.