ISOCRATIC SEPARATION OF AMPHENICOLS ON HALO PHENYL-HEXYL PHASE
HALO by AMT
Application n°57-AM
TEST CONDITIONS:
Column: 4.6 x 50 mm, HALO Phenyl-Hexyl
Part Number: 92814-406
Mobile Phase: 55/45-A/B
A= 0.025 M Ammonium acetate buffer, pH=5.8
B=Acetonitrile
Flow Rate: 1.0 mL/min.
Pressure: 94 Bar
Temperature: 35 °C
Detection: UV 240/280 nm, VWD
Injection Volume: 0.3 μL
Sample Solvent: Acetonitrile
Response Time: 0.02 sec.
Flow Cell: 2.5 μL semi-micro
LC System: Shimadzu Prominence UFLC XR
Extra column volume: ~14 μL
This separation shows a rapid HPLC method for the analysis of amphenicols on HALO Phenyl-Hexyl stationary phase. To improve the sensitivity of detection the first peak was monitored @ 240 nm and the second @ 280 nm.