I got no signal with CooBlue.
I think I performed the staining correctly (rince native PAGE gel in water for 3x5min, fix gel 15min in metoh/acide, wash for 2x10min, stain with Cooblue for 1H.)
I though the protein quantity may be to low, because my sample are 3µg of a protein lysate (?)
Do you have an idea?
thank you so much