SEPARATION OF BENZODIAZEPINES ON HALO PHENYL-HEXYL, C18, AND PFP PHASES
HALO by AMT
Application n°51-BZ
TEST CONDITIONS:
Columns: 4.6 x 50 mm, HALO Phenyl-Hexyl,
C18, and PFP
Part Numbers: 92814-406, 92814-402, and
92814-409, respectively
Mobile Phase:
A= 25 mM Ammonium acetate in water
(pH=5.8 not adjusted), B= Acetonitrile
Gradient from 34-63% B in 3.5 minutes
Flow Rate: 1.5 mL/min.
Pressure: 200 Bar
Temperature: 35°C
Detection: UV 254 nm, VWD
Injection Volume: 1.0 μL
Standard diluted with Acetonitrile and buffer
Response Time: <0.12 sec.
Flow Cell: 5 μL semi-micro
LC System: Agilent 1100
Gradient dwell volume= 0.88 mL
These separations of benzodiazepines on three different HALO Fused-Core HPLC stationary phases show the utility of having a variety of phases to optimize selectivity and/or to shorten analysis time.