ISOCRATIC SEPARATION OF DINITROTOLUENES ON HALO PFP PHASE
HALO by AMT
Application n°36-EX
TEST CONDITIONS:
Column: 4.6 x 50 mm, HALO PFP
Part Number: 92814-409
Mobile Phase: 45/55-Water/Methanol
Flow Rate: 1.5 mL/min.
Pressure: 225 Bar
Temperature: 30 °C
Detection: UV 254 nm, VWD
Injection Volume: 1.0 μL
Sample Solvent: 50/50-Methanol/Acetonitrile
Response Time: 0.02 sec.
Flow Cell: 2.5 μL semi-micro
LC System: Shimadzu Prominence UFLC XR
Extra column volume: ~14 μL
These dinitrotoluenes are difficult to separate, but can be separated with baseline resolution in under 5 minutes using a HALO PFP (perfluorophenylpropyl) column.