RAPID ISOCRATIC SEPARATION OF FIBRATES ON HALO C-18 PHASE
HALO by AMT
Application n°30-P
TEST CONDITIONS:
Column: 4.6 x 50 mm, HALO C-18
Part Number: 92814-402
Mobile Phase: 20/80-A/B
A= 0.02 M Phosphate buffer, pH=3.0
B=Acetonitrile
Flow Rate: 2.5 mL/min.
Pressure: 150 Bar
Temperature: 45 °C
Detection: UV 220 nm, VWD
Injection Volume: 0.3 μL
Sample Solvent: 50/50-methanol/acetonitrile
Response Time: 0.02 sec.
Flow Cell: 2.5 μL semi-micro
LC System: Shimadzu Prominence UFLC XR
Extra column volume: ~14 μL
Fibrates are a class of cholesterol lowering drugs that can be rapidly analyzed using Halo C-18 phase to obtain widely separated peaks in about 30 seconds.