When should I add NucView™ 488 Caspase-3 Substrate to my cells?
FAQ from Biotium
NucView™ 488 Caspase-3 Substrate can be added to the cells at the start of the experiment or at the end. A major advantage of NucView™ 488 Caspase-3 Substrate compared to other apoptosis assays is that it can be used to monitor capase-3 activity in real time.