SEPARATION OF ANTIBIOTIC AND ANTIFUNGAL DRUGS ON HALO RP-AMIDE
HALO by AMT
Application n°080-AF
TEST CONDITIONS:
Column: 4.6 x 50 mm, HALO RP-Amide
Part Number: 92814-407
Mobile Phase: Gradient
A= 0.02 M Potassium phosphate buffer, pH=3
B= Acetonitrile
Gradient:
Time %B
0.0 41
1.0 80
1.6 80
Flow Rate: 2.0 mL/min.
Pressure: 188 Bar at start of gradient
Temperature: 35°C
Detection: UV 230 nm, VWD
Injection Volume: 0.3 μL
Sample Solvent: water/acetonitrile-25/75
Response Time: 0.02 sec.
Flow Cell: 2.5 μL semi-micro
LC System: Shimadzu Prominence UFLC XR
The antimicrobial drug clofazimine and the antifungal drugs can be rapidly analyzed using the HALO RP-Amide column under gradient conditions with low back pressure.