SEPARATION OF SIX FLAVONOIDS ON HALO C18, 2.7µm
HALO by AMT
Application n°096-FL
TEST CONDITIONS:
Column: 4.6 x 50 mm, HALO C18, 2.7μm
Part Number: 92814-402
Mobile Phase: 70/30: A/B
A= 0.02 M Phosphate buffer, pH=2.9, (adj.)
B= Acetonitrile
Flow Rate: 2.0 mL/min.
Pressure: 224 Bar
Temperature: 30°C
Detection: UV 240 nm, VWD
Injection Volume: 1.0 μL
Sample Solvent: methanol
Response Time: 0.02 sec.
Flow Cell: 2.5 μL semi-micro
LC System: Shimadzu Prominence UFLC XR
ECV: ~14μL
Data rate: 25 Hz
Flavonoids are naturally occurring polyphenols that are found in plant leaves, flowers and seeds. They have beneficial health effects and are often taken as dietary supplements. Analysis of this flavonoids mixture can be carried out in less than 2 minutes using a short HALO Fused Core C18 column.