How do the Trevigen Cell Invasion Assays compare to wound healing assays?
FAQ from Trevigen
Wound healing assays, also known as scratch assays, monitor cell migration laterally on a tissue culture treated plate.
This is accomplished by generating a void in a cell monolayer by either removing cells or treating the surface of the plate to prevent cell growth in a designated area.
The assay measures the ability of the cell monolayer to fill this void, and it may be conducted in the presence of extracellular matrix proteins.
Since this assay is conducted within one chamber, there is no chemotactic gradient, and without the membrane, the cells are no longer required to change shape and squeeze through the pores.
Another potential problem is that this assay does not control for differences in cell proliferation.
While wound healing assays may be valuable for supplementing the Boyden chamber assay, it is not a replacement.