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How far is the NGG to the 3'end of the 20bp sequence?

PostPosted: Thu 9 Mar 2017 10:17
by Interchim
A 20bp target sequence is needed with a NGG PAM seq. Shall the NGG be exactly immediately following the 3' of this 20bp sequence?
FAQ from OriGene

Yes, the NGG is located immediately next to the 3' end of the 20bp sequence

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