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SEPARATION OF ANTIDEPRESSANTS ON HALO PENTA-HILIC

PostPosted: Tue 2 May 2017 16:50
by Interchim
SEPARATION OF ANTIDEPRESSANTS ON HALO PENTA-HILIC
HALO by AMT
Application n°67-AD

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TEST CONDITIONS:
Column: 4.6 x 100 mm, HALO Penta-HILIC
Part Number: 92814-605
Mobile Phase: 7/93: A/B
A= 0.1 M Ammonium formate, pH=3.5 (adj.)
B= Acetonitrile
Flow Rate: 2.5 mL/min.
Pressure: 165 Bar
Temperature: 30°C
Detection: UV 254 nm, VWD
Injection Volume: 0.5 μL
Sample Solvent: 10/90: Water/Acetonitrile
Response Time: 0.02 sec.
Flow Cell: 2.5 μL semi-micro
LC System: Shimadzu Prominence UFLC XR
ECV: ~14 μL

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Basic drugs such as antidepressants can be separated rapidly under HILIC conditions with good peak shape using HALO Penta-HILIC stationary phase.

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