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SEPARATION OF LOCAL ANESTHETICS ON HALO 2 PENTA-HILIC

PostPosted: Fri 2 Jun 2017 14:30
by Interchim
SEPARATION OF LOCAL ANESTHETICS ON HALO 2 PENTA-HILIC
HALO by AMT
Application n°119-B

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TEST CONDITIONS:
Column: 2.1 x 100 mm, HALO 2 Penta-HILIC
Part Number: 91812-605
Isocratic: 92/8:ACN/water with 5 mM
Ammonium Formate buffer, pH 3
Flow Rate: 0.5 mL/min.
Pressure: 229 bar
Temperature: 30°C
Detection: UV 245 nm, PDA
Injection Volume: 1 μL
Sample Solvent: 90/10 ACN/0.1 M
Ammonium Formate buffer, pH 3
Data Rate: 40 Hz
Response Time: 0.1 sec.
Flow Cell: 2.5 μL semi-micro
LC System: Agilent 1200 SL

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HALO 2 Penta-HILIC separates these five local anesthetics with good peak shape in less than 1.5 minutes.

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