SEPARATION OF DIOSMIN AND HESPERIDIN
Posted: Fri 19 May 2017 16:15SEPARATION OF DIOSMIN AND HESPERIDIN ON HALO PHENYL-HEXYL AND HALO RP-AMIDE
HALO by AMT
Application n°083-FL
TEST CONDITIONS:
Column 1: 4.6 x 50 mm, HALO Phenyl-Hexyl
Part Number: 92814-406
Column 2: 4.6 x 50 mm, HALO RP Amide
Part Number: 92814-407
Mobile Phase: 78/22: Water/acetonitrile
Flow Rate: 1.5 mL/min.
Pressure: 145 Bar
Temperature: 40°C
Detection: UV 254 nm, VWD
Injection Volume: 0.5 μL
Sample Solvent: Dimethylformamide*
Response Time: 0.02 sec.
Flow Cell: 2.5 μL semi-micro
LC System: Shimadzu Prominence UFLC XR
ECV: ~14 μL
These two semi-synthetic flavonoids are often taken to enhance vascular health. The two compounds may be easily separated using either HALO RP-Amide or HALO Phenyl-Hexyl phases. Note the difference in elution order on the two phases.
HALO by AMT
Application n°083-FL
TEST CONDITIONS:
Column 1: 4.6 x 50 mm, HALO Phenyl-Hexyl
Part Number: 92814-406
Column 2: 4.6 x 50 mm, HALO RP Amide
Part Number: 92814-407
Mobile Phase: 78/22: Water/acetonitrile
Flow Rate: 1.5 mL/min.
Pressure: 145 Bar
Temperature: 40°C
Detection: UV 254 nm, VWD
Injection Volume: 0.5 μL
Sample Solvent: Dimethylformamide*
Response Time: 0.02 sec.
Flow Cell: 2.5 μL semi-micro
LC System: Shimadzu Prominence UFLC XR
ECV: ~14 μL
These two semi-synthetic flavonoids are often taken to enhance vascular health. The two compounds may be easily separated using either HALO RP-Amide or HALO Phenyl-Hexyl phases. Note the difference in elution order on the two phases.
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