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SEPARATION OF SULFA DRUGS ON HALO RP-AMIDE

PostPosted: Wed 24 May 2017 12:34
by Interchim
SEPARATION OF SULFA DRUGS ON HALO RP-AMIDE
HALO by AMT
Application n°011-AB

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TEST CONDITIONS:
Column: 4.6 x 50 mm, HALO RP-Amide
Part Number: 92814-407
Mobile Phase: 70/30 A/B
A=0.1% formic acid with 0.005 M ammonium
formate (pH=3.0)
B= acetonitrile
Flow Rate: 2.0 mL/min.
Pressure: 193 Bar
Temperature: 35°C
Detection: UV 254 nm, VWD
Injection Volume: 0.5 μL
Sample Solvent: methanol
Response Time: 0.02 sec.
Flow Cell: 2.5 μL semi-micro
LC System: Shimadzu Prominence UFLC-XR
Extra column volume: ~14 μL

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