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HPLC SEPARATION OF DIURETICS ON HALO PHENYL-HEXYL

PostPosted: Fri 16 Jun 2017 15:16
by Interchim
HPLC SEPARATION OF DIURETICS ON HALO PHENYL-HEXYL
HALO by AMT
Application n°078-DU

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TEST CONDITIONS:
Column: 4.6 x 100 mm, HALO Phenyl-Hexyl
Part Number: 92814-606
Mobile Phase:
A= 0.02 M Potassium phosphate buffer, (pH=3)
B= Acetonitrile
Time %B Time %B
0 15 3.0 50
1.7 15 7.0 60
Flow Rate: 1.5 mL/min.
Pressure: 253 Bar (@start of gradient)
Temperature: 30°C
Detection: UV 230 nm, VWD
Injection Volume: 2 μL
Sample Solvent: Acetonitrile
Response Time: 0.02 sec.
Flow Cell: 2.5 μL semi-micro
LC System: Shimadzu Prominence UFLC XR
ECV: ~14 μL

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This separation illustrates the utility of HALO Fused-Core Phenyl-Hexyl phase in the rapid analysis of common diuretics.

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AMT_078-DU_Diuretics_HALO2_Phenyl-Hexyl_ITM.pdf
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