SEPARATION OF ANTIHISTAMINES ON HALO-5 C18
Posted: Fri 23 Jun 2017 14:31SEPARATION OF ANTIHISTAMINES ON HALO-5 C18
HALO by AMT
Application n°114-AH
TEST CONDITIONS:
Column: 3.0 x 100 mm, HALO-5 C18
Part Number: 95813-602
Mobile Phase: A/B: 50/50 to start.
A = 0.02 M Phosphate buffer, pH=2.6
B = Methanol
Gradient
Time %B
0.0 50
0.5 50
2.5 75
4.0 75
Flow Rate: 1.0 mL/min.
Pressure: 191 Bar
Temperature: 40°C
Detection: UV 230 nm, VWD
Injection Volume: 1.0 μL
Sample Solvent: 80% Methanol in water
Response Time: 0.02 sec.
Flow Cell: 2.5 μL semi-micro
LC System: Shimadzu Prominence UFLC XR
ECV: ~14 μL
These six antihistamines can be rapidly separated on a HALO-5 Fused-Core C18 column in under 4 minutes.
HALO by AMT
Application n°114-AH
TEST CONDITIONS:
Column: 3.0 x 100 mm, HALO-5 C18
Part Number: 95813-602
Mobile Phase: A/B: 50/50 to start.
A = 0.02 M Phosphate buffer, pH=2.6
B = Methanol
Gradient
Time %B
0.0 50
0.5 50
2.5 75
4.0 75
Flow Rate: 1.0 mL/min.
Pressure: 191 Bar
Temperature: 40°C
Detection: UV 230 nm, VWD
Injection Volume: 1.0 μL
Sample Solvent: 80% Methanol in water
Response Time: 0.02 sec.
Flow Cell: 2.5 μL semi-micro
LC System: Shimadzu Prominence UFLC XR
ECV: ~14 μL
These six antihistamines can be rapidly separated on a HALO-5 Fused-Core C18 column in under 4 minutes.
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