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SEPARATION OF STILBENES ON HALO-5 C8 AND ES-CN

PostPosted: Fri 23 Jun 2017 16:12
by Interchim
SEPARATION OF STILBENES ON HALO-5 C8 AND ES-CN
HALO by AMT
Application n°115

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TEST CONDITIONS:
Column #1: 4.6 x 50 mm, HALO-5, C8 Part (Number: 95814-408)
Column #2: 4.6 x 50 mm, HALO-5, ES-CN (Part Number: 95814-404)
Mobile Phase: A = Water, B = Acetonitrile
Gradient:
Time %B
0.0 40
3.0 60
4.0 60
Flow Rate: 2.0 mL/min.
Pressure: 120 Bar (starting)
Temperature: 30°C
Detection: UV 230 nm, VWD
Injection Volume: 1.0 μL
Sample Solvent: 50/50: water/acetonitrile
Response Time: 0.02 sec.
Flow Cell: 2.5 μL semi-micro
LC System: Shimadzu Prominence UFLC XR
ECV: ~14 μL

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These two HALO-5 phases illustrate the difference in selectivity for the cis- and trans- isomers of these stilbene compounds and the utility of different bonded phases.

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