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SEPARATION OF SULFA DRUGS ON HALO-5 C18

PostPosted: Fri 23 Jun 2017 16:24
by Interchim
SEPARATION OF SULFA DRUGS ON HALO-5 C18
HALO by AMT
Application n°108-AB

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TEST CONDITIONS:
Column: 4.6 x 50 mm, HALO-5, C18 (Part Number: 95814-402)
Mobile Phase: 87/13: A/B
A= 0.02 M Ammonium formate, pH=3 (adj)
B= 50/50: Acetonitrile/methanol
Flow Rate: 2.5 mL/min.
Pressure: 185 bar
Temperature: 30°C
Detection: UV 254 nm, VWD
Injection Volume: 1.0 μL
Sample Solvent: 50/50: Water/acetonitrile
Data rate: 50 pps
Response Time: 0.02 sec.
Flow Cell: 2.5 μL semi-micro
LC System: Shimadzu Prominence UFLC XR
ECV: ~14 μL

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This separation shows the rapid analysis of 8 sulfa drugs on HALO-5 Fused-Core, 5 micron, C18 phase. The use of mixed organic solvents improved the selectivity between compounds having similar structures.

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