HALO AQ-C18 Separation of a Nucleoside Analog and Nucleobase
Posted: Wed 11 Oct 2017 17:16
HALO AQ-C18 SEPARATION OF A NUCLEOSIDE ANALOG AND NUCLEOBASES
HALO by AMT
Application n°158-NU
TEST CONDITIONS:
Column: HALO 90Å, AQ-C18, 2.7 μm, 4.6 x 50mm
Isocratic: Water, 0.1% TFA
Flow Rate: 2.0 mL/min
Pressure: 290 bar
Temperature: 30°C
Detection: UV 254 nm, PDA
Injection Volume: 0.5 μL
Sample Solvent: Water, 0.1% TFA
Acquisition Rate: 100 Hz
Response Time: 0.05 sec
Flow Cell: 1 μL
This separation of a nucleoside analog and nucleobases on a HALO AQ-C18 column shows excellent peak shape and efficiency using 100% aqueous mobile phase conditions.
HALO by AMT
Application n°158-NU
TEST CONDITIONS:
Column: HALO 90Å, AQ-C18, 2.7 μm, 4.6 x 50mm
Isocratic: Water, 0.1% TFA
Flow Rate: 2.0 mL/min
Pressure: 290 bar
Temperature: 30°C
Detection: UV 254 nm, PDA
Injection Volume: 0.5 μL
Sample Solvent: Water, 0.1% TFA
Acquisition Rate: 100 Hz
Response Time: 0.05 sec
Flow Cell: 1 μL
This separation of a nucleoside analog and nucleobases on a HALO AQ-C18 column shows excellent peak shape and efficiency using 100% aqueous mobile phase conditions.