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HALO AQ-C18 Separation of a Nucleoside Analog and Nucleobase

PostPosted: Wed 11 Oct 2017 17:16
by Interchim
HALO AQ-C18 SEPARATION OF A NUCLEOSIDE ANALOG AND NUCLEOBASES
HALO by AMT
Application n°158-NU

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TEST CONDITIONS:
Column: HALO 90Å, AQ-C18, 2.7 μm, 4.6 x 50mm
Isocratic: Water, 0.1% TFA
Flow Rate: 2.0 mL/min
Pressure: 290 bar
Temperature: 30°C
Detection: UV 254 nm, PDA
Injection Volume: 0.5 μL
Sample Solvent: Water, 0.1% TFA
Acquisition Rate: 100 Hz
Response Time: 0.05 sec
Flow Cell: 1 μL

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This separation of a nucleoside analog and nucleobases on a HALO AQ-C18 column shows excellent peak shape and efficiency using 100% aqueous mobile phase conditions.

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Download_Application_AMT_158-NU_Interchim_201710.pdf
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