PEPTIDE PHENYL-HEXYL COMPARED TO ES-C18 AND ES-CN
Posted: Wed 11 Oct 2017 15:11
CENHANCED SELECTIVITY OF PEPTIDE PHENYL-HEXYL COMPARED TO ES-C18 AND ES-CN
HALO by AMT
Application n°159-PE
TEST CONDITIONS:
Columns:
HALO 160Å Peptide ES-C18, 2.7 μm, 2.1x150mm
Part Number: 92122-702
HALO 160Å Peptide ES-CN, 2.7 μm, 2.1x150mm
Part Number: 92122-704
HALO 160Å Peptide Phenyl-Hexyl, 2.7 μm, 2.1x150mm
Mobile Phase:
A = 0.1% formic acid in water + 10mM ammonium formate
B = 50/50 n-propanol/water + 0.1% formic acid + 10mM ammonium formate (pH: 3.45)
Flow Rate: 0.4 mL/min.
Gradient: 10-60%B in 15 min.
Temperature: 60 °C
Detection: UV 220 nm, PDA
Injection Volume: 2 μL
Sample Solvent: water, 0.1% TFA
Response Time: 0.025 sec.
Data Rate: 12.5 Hz
LC System: Shimadzu Nexera
Flow Cell: 1 μL
The initial separation using ES-C18 showed inadequate resolution of peaks 5, 6. The same separation was attempted on a ES-CN column which improved resolution of peaks 5,6 but resulted in coelution of peaks 3,4. The Phenyl Hexyl column showed excellent resolution between peaks 3,4 along with peaks 5,6.
HALO by AMT
Application n°159-PE
TEST CONDITIONS:
Columns:
HALO 160Å Peptide ES-C18, 2.7 μm, 2.1x150mm
Part Number: 92122-702
HALO 160Å Peptide ES-CN, 2.7 μm, 2.1x150mm
Part Number: 92122-704
HALO 160Å Peptide Phenyl-Hexyl, 2.7 μm, 2.1x150mm
Mobile Phase:
A = 0.1% formic acid in water + 10mM ammonium formate
B = 50/50 n-propanol/water + 0.1% formic acid + 10mM ammonium formate (pH: 3.45)
Flow Rate: 0.4 mL/min.
Gradient: 10-60%B in 15 min.
Temperature: 60 °C
Detection: UV 220 nm, PDA
Injection Volume: 2 μL
Sample Solvent: water, 0.1% TFA
Response Time: 0.025 sec.
Data Rate: 12.5 Hz
LC System: Shimadzu Nexera
Flow Cell: 1 μL
The initial separation using ES-C18 showed inadequate resolution of peaks 5, 6. The same separation was attempted on a ES-CN column which improved resolution of peaks 5,6 but resulted in coelution of peaks 3,4. The Phenyl Hexyl column showed excellent resolution between peaks 3,4 along with peaks 5,6.