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Proteinase K

PostPosted: Wed 15 Jun 2016 09:24
by Mista
Hello,

Does someone could inquire me on the Proteinase K please? I know the question seems blurry, but I would need a maximum of information.

Thank you very much,
Mista

Re: Proteinase K

PostPosted: Wed 15 Jun 2016 10:46
by Samuel
Hi Mista,

The proteinase K is a subtilisin-like endolytic protease that is isolated from the saprophytic fungus Tritirachium album. It has a high activity that is stable across a wide range of pH and temperature conditions and is suited to short digestion times. The activity of proteinase K is increased at elevated temperatures up to 65°C. Calcium is not essential to the function of proteinase K. Therefore, EDTA and other chelating agents don't interfere with the activity and may be used alongside proteinase K to inactivate calcium-dependent nucleases in DNA and RNA preparation.

Properties of Proteinase K
Alternate names: Peptidase K, Tritirachium alkaline proteinase
Specificity: Cleaves at the carboxyl side of aliphatic, aromatic or hydrophobic residues
Proteinase K Source: Tritirachium album
Molecular weight: 28,900
Form: Lyophilized form
Concentration/activity: >30 units/mg at 35°C
RNase/DNase: RNase-free and DNase-free
Protease type: Serine protease
Uses/applications: Inactivation of RNase and DNase during nucleic acid purification
Reaction conditions: 0.05-1 mg/ml proteinase K, pH 7.5-8, often containing 0.5-1% SDS
Storage conditions: Store at -20°C

Samuel

Re: Proteinase K

PostPosted: Wed 15 Jun 2016 13:54
by Mista
Thaaaaanks Samuel!