Can I label a peptide/protein/antibody other than IgG?
Posted: Mon 24 Nov 2014 13:11Can I label a peptide/protein/antibody other than IgG?
FAQ from Innova Biosciences
Yes. The Lightning-Link® technology works by targeting free amine groups on your target, meaning it can be used to label most biomolecules.
As the protocols provided were optimised for labeling IgGs, we would recommend you adjust the amount of material you add to the Lighting-Link® vial to allow for molecular weight difference. This should be done without changing the volume added to the vial, as this could affect the conjugation efficiency. As a rough guideline, we would recommend changing the amount of material proportionally to the size difference with IgGs. An average IgG is about 160kD, therefore for a target that is ½ the size of an antibody (about 80kD), add ½ as much to the vial. Please note this is only a guideline and the best amount for your assay should be determined experimentally; our 3x10µg kits enable you to do this using small amounts of material and therefore at a low cost.
Finally, please note the usual Lighting-Link® requirements will still apply
FAQ from Innova Biosciences
Yes. The Lightning-Link® technology works by targeting free amine groups on your target, meaning it can be used to label most biomolecules.
As the protocols provided were optimised for labeling IgGs, we would recommend you adjust the amount of material you add to the Lighting-Link® vial to allow for molecular weight difference. This should be done without changing the volume added to the vial, as this could affect the conjugation efficiency. As a rough guideline, we would recommend changing the amount of material proportionally to the size difference with IgGs. An average IgG is about 160kD, therefore for a target that is ½ the size of an antibody (about 80kD), add ½ as much to the vial. Please note this is only a guideline and the best amount for your assay should be determined experimentally; our 3x10µg kits enable you to do this using small amounts of material and therefore at a low cost.
Finally, please note the usual Lighting-Link® requirements will still apply