Can GelRed™ and GelGreen™ be used to stain ssDNA or RNA?
Posted: Tue 9 Dec 2014 22:29
Can GelRed™ and GelGreen™ be used to stain ssDNA or RNA?
FAQ from Biotium
GelRed and GelGreen can be used to stain both ssDNA and RNA, but GelRed is about 5 times more sensitive for single-stranded nucleic acids than GelGreen. Titration assays using a fluorescence microplate reader showed that the fluorescence signal of GelRed bound to ssDNA and RNA is about half that of GelRed bound to dsDNA.
FAQ from Biotium
GelRed and GelGreen can be used to stain both ssDNA and RNA, but GelRed is about 5 times more sensitive for single-stranded nucleic acids than GelGreen. Titration assays using a fluorescence microplate reader showed that the fluorescence signal of GelRed bound to ssDNA and RNA is about half that of GelRed bound to dsDNA.