GelRed and GelGreen compatible with downstream applications?
Posted: Wed 10 Dec 2014 11:39Are GelRed™ and GelGreen™ compatible with downstream applications such as cloning, ligation and sequencing?
FAQ from Biotium
Yes. We recommend Qiagen or Zymoclean gel extraction kits or phenol-chloroform extraction to remove the dye from DNA. Some users have reported performing PCR on DNA in the presence of GelRed with no purification step, for example by incubating GelRed-stained gel slices in TE buffer to extract DNA by passive diffusion for use in PCR, or by using a few microliters of molten agarose from GelRed-stained gel slices containing DNA for PCR.
FAQ from Biotium
Yes. We recommend Qiagen or Zymoclean gel extraction kits or phenol-chloroform extraction to remove the dye from DNA. Some users have reported performing PCR on DNA in the presence of GelRed with no purification step, for example by incubating GelRed-stained gel slices in TE buffer to extract DNA by passive diffusion for use in PCR, or by using a few microliters of molten agarose from GelRed-stained gel slices containing DNA for PCR.