Using fewer cells than suggested in the protocol ?
Posted: Fri 6 Feb 2015 18:58I am using fewer cells than suggested in the protocol, should I adjust the amount of CF488A- or FITC-Annexin V and Ethidium Homodimer III in the assay?
FAQ from Biotium
No, the concentration of Annexin V and Ethidium Homodimer III should be kept constant regardless of cell number. However, the staining concentrations can be increased or decreased if necessary to optimize staining.
FAQ from Biotium
No, the concentration of Annexin V and Ethidium Homodimer III should be kept constant regardless of cell number. However, the staining concentrations can be increased or decreased if necessary to optimize staining.