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Why does the blue label appear very weak?

PostPosted: Mon 16 Feb 2015 15:01
by Interchim
Why does the blue label appear very weak?
FAQ from Trevigen

The blue label could appear very weak, because of 2 reasons :
1/ Insufficient wash steps.
Make sure to wash in deionized water before and after the TACS Blue Labelâ„¢ step.

2/ Labeling reaction time was insufficient.
View under microscope to determine proper incubation period with TACS Blue Labelâ„¢ (2-7 minutes).