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Negative controls show more DNA damage than expected ...?

PostPosted: Mon 16 Feb 2015 15:39
by Interchim
Why are negative controls showing more DNA damage than expected in adherent cells?
FAQ from Trevigen

For trypsin incubations, incubate in 2% cold Trypsin for 30 minutes.
Centrifuge for 10 minutes at speeds less than 200xg to avoid damage. Cold EDTA (2 mM) can be used instead of Trypsin.