Negative controls show more DNA damage than expected ...?
Posted: Mon 16 Feb 2015 15:39
Why are negative controls showing more DNA damage than expected in adherent cells?
FAQ from Trevigen
For trypsin incubations, incubate in 2% cold Trypsin for 30 minutes.
Centrifuge for 10 minutes at speeds less than 200xg to avoid damage. Cold EDTA (2 mM) can be used instead of Trypsin.
FAQ from Trevigen
For trypsin incubations, incubate in 2% cold Trypsin for 30 minutes.
Centrifuge for 10 minutes at speeds less than 200xg to avoid damage. Cold EDTA (2 mM) can be used instead of Trypsin.