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Separation of nucleotide triphosphate

PostPosted: Thu 19 May 2016 11:23
by Miella
Hi !

I would like to separate nucleotides triphosphate (rNTP) by HPLC but I don't know what column to use, maybe you can help me ?

Thanks in advance !

Re: Separation of nucleotide triphosphate

PostPosted: Thu 19 May 2016 14:13
by Lolo
Hi !

I can do this on a Uptisphere ODB C18 3µm 100x4,6mm
80/20 - 5 mM TBA-Br, 20mM (NH4)2HPO4 / Acetonitrile
Débit : 1.0 mL/min, 37 °C
UV at 225 nm

Hilic seems to be a good alternative to avoid ion pair reagents
Uptisphere CS Evolution HIT-Hilic can be used for me

Re: Separation of nucleotide triphosphate

PostPosted: Thu 19 May 2016 14:50
by Miella
Hi Lolo the hero :lol:

Thank you for your answer ! :D