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Hii,

Hello
I'm interested by a Primesep 100.
Before to place an order, I would like a technical sheet about this column with the method of rincing in strongly acid or basic medium.
Which are prohibitions to avoid on this column?

Thanks,
Minnie

Hello Minnie,

If you stay with recommended pH range you should not have any problems.
Some of the basic compounds (2 or more basic groups) can interact strongly with the column and you will need to wash them off with higher buffer concentration and higher ion concentration. Columns are compatible with 100% ACN and 100% water, but when you do gradients don't go above 70% ACN and always look on how much ions you have in the mobile phase.
Do not use alcohols in mobile phase as you will esterify carboxylic acid on the surface of silica.

Have a good day
Sean

Hi Sean,
Thanks for your answer.
I have other questions about this column Primesep 100.
Could you please confirm me the pH recommended for the column and what represent "higher buffer concentration and higher ion concentration" in the case of products strongly fixed at the column ?
I will work on phenylalanine and the phenylpyruvate.
I would like to work under conditions of pH > 8 to be in prevalent form anion. What do you think about that ?
I would like to work with the couple (HPO4) 2 -/(PO4) 3 - and stay at pH 11. What do you think about that ?

Thank you,
Minnie

No, Primesep 100 can not be used above pH 7.0 similar to many other silica based columns!
Instead of high pH if someone works with compounds like phenylalanine and phenylpyruvate in order to get single ion form for both compounds they need to go to lover pH 1.5-4 and get phenylalanine positively charged and phenylpyruvate neutral.
Both compounds will be strongly retained on Primesep 100 column. The phosphate buffer still can be used in pH 2-3 range and it offers good UV transparency. Buffer concentration can be as high as 100 mM or even more if it does not cause pumping and precipitation issues. Our typical high buffer concentration in almost all the method we developed is 50 mM.

High day !
Sean

Thanks sean, I go tell all this to Minnie

Mickey