What are the sample capacities of TSKgel HIC columns?
FAQ from TOSOH
Resolution and peak width are dependent in part on how much sample is loaded on the HIC column. In general the following guidelines for sample loading can be applied:
- Ether-5PW 5 -10 mg of sample protein
- Phenyl-5PW 5 -10 mg of sample protein
- Butyl-NPR 100 ug of crude protein