Why does the blue label appear very weak?
FAQ from Trevigen
The blue label could appear very weak, because of 2 reasons :
1/ Insufficient wash steps.
Make sure to wash in deionized water before and after the TACS Blue Label™ step.
2/ Labeling reaction time was insufficient.
View under microscope to determine proper incubation period with TACS Blue Label™ (2-7 minutes).