Why is TACS Blue Label™ fading?
FAQ from Trevigen
Chlorine in tap water dissolves the blue label hence it is essential to use deionized water.
Ensure proper dehydration through decreasing alcohol series (ethanol or denatured ethanol only), and o- or p-xylenes only (no mixed xylenes). Make sure to change solutions frequently.
Use correct mounting medium. Trevigen NF Mounting Media (cat# 4865-25) is the recommended mounting medium. The fading could also be due to benzene solubility. Benzene contaminants are found in some mixed xylenes. Use o- or p-xylenes for clarification after dehydration. Do not dilute mounting medium with mixed xylenes.
Slides should be stored in the dark to maintain optimal staining.