Mouse monoclonals against Rat immunoglobulins

Mouse IgG1k - clone MARG2b-3

Mouse IgG1k - clone MARG2b-8

Mouse IgG1k - clone MARG2b-3

Immunogen :

IgG2b (IR863) (1)

Reactivity :

Rat gamma 2b heavy chain of immunoglobulin (determined by immunodot)
The specificity of MAbs anti-IgG subclasses is determined in a range of optimal concentrations. Increasing of the first or second antibody at an inadequate and unnecessary concentration can induce possible cross-reactions with other subclasses.

Avidity :

on rat IgG2b: 3 x 109 M-1 (Cf. Avidity sheet, for more details).

Applications :

Cf. reactivities (4)
- can be used as second antibody in immunoassays
- can be used for capture elisa: good binding on plastics
- can be labelled with biotin, peroxidase and FITC
- can be coated on nitrocellulose (dot-elispot)
- can be used on cryosection (immunohistology) as first antibody labelled with peroxidase or FITC
- cannot be frozen and thawed many times. susceptible to be denatured by such a treatment.

Lyophilization :

not tested

References

(1) Bazin H., Beckers A. & Querinjean P. Three classes and four subclasses of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c. Eur. J. Immunology 1974,4:44-48

(2) Pluschke G. and Bordmann G. Isolation of rat immunoglobulin class switch variants of rat-mouse hybridomas by enzyme-linked immunosorbent assay and sequential sublining. Eur. J. Immunol. 1987, 17, 413-416

(3) Manouvriez P., Lefebvre M., Genart C., Cormont F. & Bazin H. Accurate determination of rat immunoglobulin isotype content in culture supernatants or media by Elisa with mouse monoclonal antibodies. Immunobiology 1986, 173, 307-308.

(4) Bazin H., Malache JM. Rat (and mouse) monoclonal antibodies. V. A simple automated technique of antigen purification by immunoaffinity chromatography. J. Immunol. Meth., 1986, 88:19-24

Selected References of scientific publication in which MARG2b-3 was used: Soares M., et al. Transplant. Proc. 1995, 27: 282-285.

Mouse IgG1k - clone MARG2b-8

Immunogen :

IgG2b (IR863) (1)

Reactivity :

Rat gamma 2b heavy chain of immunoglobulin (determined by immunodot)
The specificity of MAbs anti-IgG subclasses is determined in a range of optimal concentrations.Increasing of the first or second antibody at a an inadequate and unnecessary concentration can induce possible cross-reactions with other subclasses.

Avidity :

on rat IgG2b: 4.7 x 109 M-1 (Cf. Avidity sheet, for more details).

Applications :

Cf Reactivity
- can be used for capture elisa: weak binding on plastics
- can be labelled with peroxidase, and biotin
- cannot be labelled with FITC
- can be used as second antibody in immunoassays (2,3)
- cross-reacts non specifically with nearly 15% of human lymphocytes (as detected by FACScan) and with human monocytes (> 35%)
- can be used in mmunoaffinity chromatography for purification of rat IgG2b (Solid phase Sepharose 4B CNBr act.)
- cannot be coated on nitrocellulose (dot-elispot)

Lyophilization :

Yes, with a possible low denaturation.

References

(1) Bazin H., Beckers A. & Querinjean P. Three classes and four subclasses of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c. Eur. J. Immunology 1974,4:44-48

(2) Pluschke G. and Bordmann G. Isolation of rat immunoglobulin class switch variants of rat-mouse hybridomas by enzyme-linked immunosorbent assay and sequential sublining. Eur. J. Immunol. 1987, 17, 413-416

(3) Manouvriez P., Lefebvre M., Genart C., Cormont F. & Bazin H. Accurate determination of rat immunoglobulin isotype content in culture supernatants or media by Elisa with mouse monoclonal antibodies. Immunobiology 1986, 173, 307-308.

Selected References of scientific publications in which MARG2b-8 MAb was used: Castedo M. et al. Int. Immunology 1993, 5: 1569-1576; Soares M. et al. 1993, 56: 1427-1433; Vanhove B., Bazin H. Mol. Immunology, 1992, 29:1-8; Reding et al. Transplant. Immunol. 1994, 2:231-237

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