Quantitative Competitive PCR Kits

The use of RT-PCR to examine levels of gene transcripts has become very popuplar because PCR is very sensitive and rapid. However, quantification of mRNA can be difficult due to the exponential nature of PCR, where small variations in amplification efficiency lead to dramatic changes in product yields. In addition, amount of product generated reaches a plateau during later stages of PCR. These characteristics can obscure differences in levels of the target mRNA during amplification.

Several methods have been described that use some of internal control to compare the efficiency of PCR in different reactions or in same tube. The internal control can be a homologous DNA or non-homologous DNA that contain priming sites and thus will compete with the target DNA for the same primers.

Maxim has developed PCR competitor, a series of DNA fragments for use as competitive internal controls in PCR. PCR competitor can be used to accurately measure small changes in RNA levels. To perform competitive PCR, known quantities of PCR competitor are added to PCR reaction containing test samples. A comparison of PCR products can be used to determine the quantity of target gene products.

Maxim's quantitative competitive RT-PCR kits (QCRT-PCR) combine its core MPCR technology with PCR competitors available. This ready-to-use kit contains all reagents except Taq Pol. to perform 100X 50 ul QCRT-PCR. Custom QCRT-PCR is also available from Maxim Biotech.

Lists Available & Ordering Information

TOP

Cat.#

Description

Qty

T65880

Human bcl-2 gene QCRT-PCR kit

100 Rxns

T65890

Human bcl-xL gene QCRT-PCR kit

100 Rxns

T65900

Human bcl-xS gene QCRT-PCR kit

100 Rxns

T65910

Human BiP Protein (GRP78) QCRT-PCR kit

100 Rxns

T65920

Human CCR-5 gene QCRT-PCR kit

100 Rxns

T65930

Human CD154 Gene (CD 40 Ligand) QCRT-PCR kit

100 Rxns

T65940

Human CD40 Gene QCRT-PCR kit

100 Rxns

T65950

Cytomegalovirus Matrix Protein (pp65) QCRT-PCR kit

100 Rxns

T65960

Human CXCR-4 gene QCRT-PCR kit

100 Rxns

T65970

Human Epstein-Barr Virus (EBV), BNRF1 QCRT-PCR kit

100 Rxns

T65980

Human Fas gene QCRT-PCR kit

100 Rxns

T66000

Human basic FGF QCRT-PCR kit

100 Rxns

T66060

Human caspase-3 gene QCRT-PCR kit

100 Rxns

T66070

Human caspase-8 QCRT-PCR kit

100 Rxns

T66080

Human caspase-9 QCRT-PCR kit

100 Rxns

T66090

Human IFN-r gene QCRT-PCR kit

100 Rxns

S08950

Human IL-10 gene QCRT-PCR kit

100 Rxns

T66100

Human IL-12 gene QCRT-PCR kit

100 Rxns

T66110

Human IL-1a gene QCRT-PCR kit

100 Rxns

T66120

Human IL-1b gene QCRT-PCR kit

100 Rxns

T66130

Human IL-2 gene QCRT-PCR kit

100 Rxns

T66150

Human IL-4 gene QCRT-PCR kit

100 Rxns

T66170

Human LIF Human LIF QCRT-PCR kit

100 Rxns

S23170

Human iNOS gene QCRT-PCR kit

100 Rxns

T66210

Human TGF-b gene QCRT-PCR kit

100 Rxns

S08980

Human TNF-a gene QCRT-PCR kit

100 Rxns

AU2530

Human Low Density Liproprotein-related Protein 1 (LRP1) QCRT-PCR kit

100 Rxns

S08960

Mouse IL-10 gene QCRT-PCR kit

100 Rxns

T66140

Mouse IL-2 gene QCRT-PCR kit

100 Rxns

T66220

Mouse TGF-b gene QCRT-PCR kit

100 Rxns

T66230

Rat TGF-b gene QCRT-PCR kit

100 Rxns

T66240

Rat TNF-a gene QCRT-PCR kit

100 Rxns

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Tel. (33) (0) 4 70 03 73 06 - interbiotech@interchim.com - http://www.interchim.com