Uptima Universal Real Time PCR Master Mix Kit

FEATURES

• Contain TEMPase enzyme for increased specificity and product yield

• Designed to diminish the formation of non-specific product

• Detection of low target copy number

• 2x Master Mix in one tube. Add primers, probe and template

Description

The Uptima Universal Real-Time PCR Master Mix is a single-tube 2X reagent ideal for most quantitative real time PCR applications. The Uptima Real Time PCR kit support quantitative amplification and detection with multiplex capability and show consistent high performance with various fluorescent detection systems including Green DNA I detection, TaqMan probes and Molecular beacons. The Uptima Real Time PCR kit has been designed for optimal performance on ABI PRISM^TM Instruments, the LightCycler^TM Instrument, the Mx4000^TM Instrument and the DNA Engine Opticon^TM System. The Uptima Real Time PCR kit includes the components necessary for performing PCR amplification, and have been successfully used for amplify and detect a variety of DNA target such as genomic DNA, cDNA and plasmid DNA.

To prevent template cross-contamination the master mix are design for future UNG (Uracil-N-glycosylase) treatment by replacement of dTTP with dUTP, however the master mix does not contain the UNG enzyme in present kit version.

*The kit contains*:

2x Uptima Real Time PCR Master Mix, Reference Dye, Green Dye I, MgCl2 and glass Blocking Agents.

*Sufficient for 200 reactions of 50 µl pr. reaction*

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