TranSignal™ Target Gene Arrays: p53, NFkB, Egr, & Interferon-Inducible

• Targeted - profile the expression patterns of genes regulated by p53, NFkB, Egr, or interferon

• Fast  - immediately link your gene of interest to target gene-associated pathways

• Safe - no radioactivity required

TranSignal™ Target Gene Arrays are designed specifically for profiling the expression pattern of genes that are regulated by p53, NFkB, Egr, or interferon (IFN). With the TranSignal p53, NFkB, and Egr Target Gene Arrays, you can quickly and easily determine which p53-, NFkB-, or Egr-target genes are induced or repressed in your particular cell line or tissue sample. With the TranSignal IFN-Inducible Target Gene Array, you can profile expression of IFN-inducible genes. You can also use these arrays to identify p53-, NFkB-, Egr-, or IFN-associated pathways.

We currently offer TranSignal Target Gene Arrays for human and mouse, human and mouse NFkB, human Egr, and mouse IFN.

• p53: The Last Gatekeeper

• NFkB: A Central Player in Immunity

• Egr-1: An Immediate-Early Response Protein

• IFN: Cancer and Beyond

Selection Guide

p53: The Last Gatekeeper

The p53 protein plays a key role in maintaining ordered proliferation, growth, and differentiation of normal cells. Mutation of the gene that encodes this protein is the most frequently encountered genetic event in human malignancy. Inactivation of the p53 gene through mutation disrupts the cell cycle, which, in turn, sets the stage for tumour formation. As a transcription factor, p53 regulates the expression of various target genes by interacting with a specific cis-element found in many promoters and enhancers.

Just a few examples of p53 target genes include:

• p21 (inhibits CDK)

• Bax (mediates apoptosis)

• Type I IGF receptor (inhibits tumour growth)

Egr-1: An Immediate-Early Response Protein

Egr-1 is functionally implicated in cell proliferation, cell differentiation, neuronal plasticity, vascular wound response, cancer progression, and other important biological processes. Discovered independently by a number of laboratories searching for factors regulating cell growth and proliferation, Egr-1 is also known as nerve growth factor induced-A (NGFI-A), krox-24, ZIF268, and TIS8. Egr-1 is an 80- to 82-kDa protein consisting of 533 amino acids, containing three DNA-binding zinc-finger motifs.

Egr-1 is rapidly and transiently induced by a number of growth factors, cytokines, and injury-related stimuli. As a transcription factor, Egr-1 alters gene transcription through DNA binding mechanisms dependent on coactivators and corepressors. Thus, Egr-1 regulates and is regulated by a wide variety of other proteins.

Just a few examples of Egr-1 target genes include:

• CREB-binding protein (CBP)

• NFkB

• NGFI-A-binding proteins 1 and 2

IFN: Cancer and Beyond

The interferons (IFNs) are a large family of multifunctional secreted proteins. IFNs regulate cell growth, defend against viruses, and activate the immune system. IFNs can also play a key role in cancer therapeutics; hence, these proteins are used to treat a number of solid tumours and blood malignancies.

IFNs are classified as two distinct types. Although Type I and II IFNs share no obvious structural homology, there is a broad overlap in the types of genes they induce. IFNs induce transcription of a number of genes, including:

• Ceacam I

• Eln

• Tnf

Here's how it works:

1. Start by isolating RNA from your experimental sample and a control sample

2. Reverse-transcribe in the presence of biotin-dUTP (labeling mix is provided), then hybridise these biotin-labeled cDNA probes to TranSignal cDNA array membranes

3. Visualise results using x-ray film or chemiluminescence detection

All-in-one system, no additional materials required!

Each TranSignal Target Gene Array Kit is supplied with two array membranes; hybridization and wash solutions; complete reaction and detection reagents, including Streptavidin-HRP Conjugate, and control RNA. Additional reagents for re-probing array membranes can be purchased separately (TranSignal Target Gene Refill Kits).

References:

1. DeRisi, J.L. and Iyer, V.R. (1999) Genomics and array technology. Cur. Opin. Oncol 11: 76-79.

2. Gieser, P. et al. (2002) Introduction to microarray experimentation and analysis. Methods Mol Biol. 184: 29-49.

3. Macgregor, P. and Squire, J.A. (2002) Application of microarrays to the analysis of gene expression in cancer. Clin Chem. 48: 1170-1177. 

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