Knock-down siRNA Kit

Small interfering RNAs (siRNAs) are double stranded RNAs that the mediators of mRNA degradation in the process of RNA interference (RNAi). RNAi has been used as a tool to study gene functions (for a review, see Sharp 2001, Genes Dev. 15: 485-490). The siRNA appears that longer dsRNA is processed into 21-23 nt dsRNA (siRNA) by an enzyme, Dicer (Bernstein et al. 2001, Nature 409: 363-366; Knight SW and Bass BL 2001, Science 2: 2269-2271). Spring's Knock-down siRNA Kit provides every things you need for generation of siRNAs from the DNA fragment of target genes. Customers who have the constructs of target genes in vectors containing any two of the promoters, T7, T3, and/or SP6 at the 5'-end and 3'-end of inserted gene can directly generate siRNAs with this kit within one day.

 

Spring's Knock-down siRNA Kit Procedure:

Spring's Knock-down siRNA Kit has the following advantages:

  • Flexible

    This well-designed kit allows you to generate siRNA in your own laboratory.

  • Efficient

    Because this kit starts with a DNA fragment of the target gene, it can generate a mixture of siRNAs that has much more chance to knock-down the target gene than a single or few siRNAs.

  • Cost Effective

    One kit can be used for five genes knock-down experiments and more than 100 tranfections.

INTERCHIM DNA Microarray

Microarray and gene expression studies

Slides and Reagents for self-printing microarrays

Hybridization

Calibration & Software

Complementary Studies

 

 

Product Type

Sub Type

Catalog #

Size

Knock-down siRNA Kit 1

T7 Transcription Kit, T7 Trans.Kit siRNA Kit

AM6950

5 rxns

Knock-down siRNA Kit 2

SP6 Transcription Kit, SP6 Trans.Kit siRNA Kit

AM6970

5 rxns

Knock-down siRNA Kit 3

T3 Transcription Kit, T3 Trans.Kit siRNA Kit

AM6990

5 rxns

Knock-down siRNA Kit 4

T7 Transcription Kit, SP6 Trans.Kit siRNA Kit

AM7020

5 rxns

Knock-down siRNA Kit 5

T7 Transcription Kit, T3 Trans.Kit siRNA Kit

AM7030

5 rxns

Knock-down siRNA Kit 6

SP6 Transcription Kit, T3 Trans.Kit siRNA Kit

AM7040

5 rxns

siRNA Kit

Dicer Blend etc.

AM7050

5 rxns

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