Our FluoProbes 647 NHS provide an high efficiency for labeling. Compared to the competitors 647, FluoProbes 647 has been used successfully to increase the quantity of dye linked to the target (more than 10% of dye after labeling). Also FluoProbes 647 shows extended signal emission time. In regards of its competitive price, it will be the right choice for routine application with a single dye like Cy5.
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Description |
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Dye |
FluoProbes 630 |
FluoProbes 647 |
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Absorption maximum [nm] |
636 |
652 |
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Emission maximum [nm] |
657 |
673 |
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Molar absorbance [l·mol-1·cm-1] |
200.000 |
250.000 |
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Molecular weight [g·mol-1] |
731,92 |
761,85 |
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Researchers Discover the Advantages of Making the Switch
Quality control of chip manufacture and chip analysis using epoxy-chips as a model
C. Preininger and U. Sauer, Elsevier, Sensors and Actuators B 6882 (2003) 1–6 (extract)
[…] We tested an alternative fluorescent label: FluoProbes 630-NHS is based on a bridged hemicyanine dye and was designed for excitation by He/Ne- and diode-lasers. To compare the extent of incorporation of Cy5 and FluoProbes 630, 0.3, 0.5 and 1 ml extracted Rhizobium fredii DNA were used as template for the PCR-reaction. As can be seen from (Fig.1), FluoProbes 630 led to a higher yield of labeled DNA. Thus, from the same starting amount of DNA amplified DNA with a higher level of label incorporation and therefore higher fluorescence can be produced. As a result, less DNA is needed for chip analysis which especially in medical diagnostics and cancer research, where probe material is very limited, is of great importance. […]
 Fig.1:
Bands
of amplified Rhizobium fredii, when using 0.3, 0.5 and 1 mg extracted
DNA as a template for the PCR-reaction. |
DNA microarray analysis of the hyperthermophilic archaeon Pyrococcus furiosus: evidence for the new type of sulfurreducing enzyme complex. Schut G.J., Zhou J., and Adams, M.W., J Bacteriol 183, 7027 (2001).
In this paper, the authors break new ground in microarray analysis, from both a scientifi c and a technical perspective. They present the fi rst microarray study of the metabolism of an archaeon; additionally, they describe one of the fi rst microarray studies to use three samples, each labeled with a different dye, hybridized simultaneously to the same microarray. The hyperthermophile Pyrococcus furiosus thrives at 95°C, using either carbohydrates or peptides as carbon sources. When using peptides as a carbon source, the metabolism is aided by the reduction of elemental sulfur (S°) to H2S. It is already known that the presence of S° in the medium alters the activities of key metabolic enzymes in these organisms. Using microarray analysis, the authors show that much of the enzyme activity is altered at the transcriptional level — enzymes in several different hydrogenase systems are either upregulated or downregulated in the presence of S°. It will be exciting to discover the mechanism by which elemental sulfur regulates gene transcription. To perform their experiments, the authors made the switch from Cy dyes to competitor Fluor dyes, allowing them to examine three samples on a single microarray. They labeled their cDNA using the ARES DNA Labeling Kits. The competitor 488, competitor 546 and competitor 594 dyes matched perfectly with the laser lines and fi lters in their ScanArray 5000 scanner (Packard).
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INTERCHIM DNA Microarray Microarray and gene expression studies |
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