GENORAMATM Microarray Slides

GenoramaTM Microarray Slides are developed and tested primarily for DNA-based applications. However, RNA and proteins can also be immobilized.

The activated glass slides are produced under rigorous quality control and tested for low background and good immobilization characteristics. As a result, the slides have a very clean and uniform surface, highly suitable for printing top quality microarrays. The microarray slides have a double-sided coating with a printable area of 23 x 73 mm.

Coated slides are packed in boxes of 25 and sealed in an alufoil bag to protect the activated surface from light and maintain a stabile storage environment.

The protocols of the stringent production procedures have been developed in Asper Biotech Ltd. and the process is certified to be in accordance with ISO9001 standards.

Asper also provides bar-coded slides upon request.

INTERCHIM DNA Microarray

Microarray and gene expression studies


Slides and Reagents for self-printing microarrays

Hybridization

Calibration & Software

Complementary Studies

 

Slides and Reagents for self-printing microarrays

TWO SURFACE COATINGS ARE AVAILABLE

Type

SA

SAL

Coating

Aminosilane

Aminosilane with linker

For binding

Unmodified DNA, long aminated DNA

Short aminated DNA

Type of binding

Noncovalent bond

Covalent bond

Optimized for

Gene expression

Genotyping

Buffer

GenoramaTM Spotting Solution II

GenoramaTM Spotting Solution I

Two sizes of the slides are produced.
Standard slides 25 x 75 x 1 mm and thin slides 24 x 60 x 0.15 mm.

 

Code

Coating

W x L x H mm

For binding unmodified DNA

SA -1

3-Aminopropyltrimethoxysilane

25x75x1.0

SA -0.1*

3-Aminopropyltrimethoxysilane

24x60x0.15

For binding aminated DNA

SAL -1

3-Aminopropyltrimethoxysilane + 1,4-Phenylenediisothiocyanate

25x75x1.0

SAL -0.1*

3-Aminopropyltrimethoxysilane + 1,4-Phenylenediisothiocyanate

24x60x0.15

* 24 x 60 mm slides have one specially refined edge to ensure maximum uniformity of light distribution via total internal reflection excitation in the Genorama 003 Imaging System.Slides are packed in boxes of 25.

Printed slides can be stored at 4°C for up to 6 months.

 

PROBE ATTACHMENT TO SiO2 SURFACE

SA: Unmodified oligos bind to aminosilane coated slide electrostatically. Oligos can be attached either at their 5' termini or via internal phosphate groups (backbone).

SAL: Aminated DNA attaches via 5' termini to 3-Aminopropyltrimethoxysilane + 1,4-Phenylenediisothiocyanate coated glass surface by formation of covalent bond.

 

For binding unmodified DNA

Standard slides

SA –1

Standard slides, barcode

SA –1

Thin slides

SA -0.1

Thin slides, barcode

SA -0.1

For binding aminated DNA

Standard slides

SAL –1

Standard slides, barcode

SAL –1

Thin slides

SAL -0.1

Thin slides, barcode

SAL -0.1

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Tel. (33) (0) 4 70 03 73 06 - interbiotech@interchim.com - http://www.interchim.com