FEATURES and BENEFITS
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Choose from labeled dUTP, UTP and OBEA-dCTPs
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Fluorescent and nonfluorescent labels, including biotin, DNP, conventional dyes and Molecular Probes’ proprietary dyes (Table 1)
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Built-in spacers for reduced interference of the dye in enzymatic uptake
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Protocols optimized for reverse transcription
3. BioTechniques 30, 202 (2001).
Table 1: ChromaTide labeled nucleotides
|
Label |
Ex/Em * |
dUTP |
OBEA-dCTP |
UTP |
|
Biotin |
NA |
- |
- |
|
|
dinitrophenyl (DNP) |
NA |
- |
- |
|
|
Cascade Blue |
400/420 |
- |
- |
|
|
fluorescein |
495/520 |
- |
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|
Oregon Green 488 |
490/520 |
- |
- |
|
|
- |
- |
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* Approximate excitation (Ex) and emission (Em) maxima, in nm. T. Tested and found suitable for incorporation by reverse transcriptase. NA = Not applicable. |
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A microarray hybridized . cDNA was labeled by reverse transcription from Vibrio cholerae total RNA using ChromaTide (C-11404). Labeled cDNA was then hybridized to a V. cholerae O1 El Tor microarray. The array was imaged with a ScanArray 5000XL scanner (Packard BioScience). Contributed by Kimberly Chong and Gary Schoolnik, Stanford University. |
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INTERCHIM DNA Microarray Microarray and gene expression studies |
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