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15% OFF on all Protein Modification antibodies from Abgent
Post-translational modification of the proteome is dynamic, in tune to the ever-changing needs of the cell. These modifications modulate protein function by affecting expression, stability, localization, activation and binding properties. Significantly, 5-10% of the genome encodes protein-moficiation catalizing enzymes (kinases, phosphatase, glycosyl-transferase, etc…).
There are more than 200 known protein modifications that occur in cells. Permutations of these modifications provide instant command and control of cellular function. Improper modifications are associated with major human diseases such as cancer, autoimmune diseases and neurological disorders
• Sumo1,2,3 • Phospho-specifics • Ubiquitin • SMURF1 antibodies 
See full antibody list
Sumo1 antibody example : AP1222a
The anti-SUMO1 polyclonal antibody
(Cat. #AP1222a) is used in Western blot to detect GST-SUMO1 fusion protein.
COS-7 cells were transfected for 24 hrs with a plasmid expressing FLAG-ERM (left panels) or FLAG-ERM KR12345 (right panels). Untreated (-) and H2O2-treated (+) cells were collected for immunoblot analysis. Top panels: cell lysates probed by western blot (WB) with an anti-ERM antibody. Center panels: cell lysates immunoprecipitated (IP) with an anti-FLAG antibody followed by WB with AP1222a SUMO-1 antibody. Bottom panels: cell lysates immunoprecipitated with an anti-FLAG antibody followed by WB with AP1224a SUMO-2/3 antibody. (*) represents immunoprecipitated ERM-like forms recognized by anti-SUMO antibodies.
Product citations :
1- Rathke, Christina, et al. J. Cell Sci. 2007 May 01;120(9):1689-1700. (Applications: IF)
2- Degerny, Cindy, et al. J. Biol. Chem. 2005 Jul 01;280(26):24330-24338. (Applications: WB)
Find all other antibodies from Abgent in SAMPLE SIZE at : 85€HT / 20µg (*)
Sample size available on all items with cat# AP... or AM..., ie over 5000 antibodies !
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