Cell Biology Tools

Cell culture reagents

Antibiotic and Antifungic agents

 

 

Ampicillin

UP391961

25g

Technical information

  • The culture media, prepared or purchased, are classically autoclaved, cooled to 50°C, then supplemented with antibiotic solutions.

  • Antibiotic solutions may too be applied by vaporisation directly onto agar petri dishes, or included in agar gel preparation.

  • Most vectors, both DNA plasmides and phages, and hybridoma, contain genes encoding for antibiotic resistance. The resistance identifies the vectors, and confer the resitance to the hosting cell (bacteria or mamalian cell), and permit the positive selection of transfected cells: antibiotic and antifungic agents block, or kill undesired organism, but don’t affect the cultivated cell.

Carbenicillin, disodium salt

 UP383880 1g

Chloramphenicol

 UP091421 100g

Kanamycin sulfate

 UP308664 10g

Streptomycin sulfate

 UP224968 100g

Tetracyclin hydrochloride

 UP259169 100g

Applications

In any invitro culture, it is essential to remove undesired bacterial and fungal strains that were present in the inoculum, or come by contamination.

  • In vitro culture of animal cells

  • In vitro culture of bacteria

  • In vitro culture of hybridoma (monoclonal antibody production)…

  • In vitro culture of vectors (recombinant protein production)

  • Extraction and purification of proteins

  • Long term Dialysis

  • Preservative for reagents

Uptima antibiotic and antifungic are produced according state of art procedures to ensure optimal results in cell culture, as well for protein preservation.

Without further indication, antibiotics may be prepared in filter-sterilized distilled water, and stored frozen at –20°C.

 

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