Molecular Biology

molecular weight markers

 

 

Nucleic molecular size markers

All Nucleic Acid MW markers are supplied in 50 µg quantity (0.5 µg/µl) allowing 100 lanes analysis.

100 bp DNA Ladder

UPS54811

50µg (100rxns)

1 Kbp DNA Ladder

UPS54802

50µ (200 rxns)

The 100 bp DNA ladder is suitable for sizing linear double-stranded DNA fragments from 100 to 1000 bp. The 10 bands of the ladder contain fragments with the following sizes:
100, 200, 300, 400, 500, 600, 700, 800, 900 and 1000 bp

The recommended amount of size marker to load on an agarose gel is 0.5-1 µg per lane (5-10 µl).

To demonstrate the mobility of the DNA fragments, 1 µg of marker were loaded onto a 1% agarose gel.

Concentration: 1 µg/10 µl

Pack size*: 50 µg in 500 µl (100 rxns) loading buffer (0.25% bromphenoblue, 0.25% xylene cyanol FF, 40% sucrose in water).

The 1 kb DNA ladder is suitable for sizing linear double-stranded DNA fragments from 0.25 to 10 kb. The 13 bands of the ladder contain fragments with the following sizes:
250, 500, 750, 1000, 1500, 2000, 2500, 3000, 4000, 5000, 6000, 8000 and 10000 bp For easy reference on agarose gels, the 1000 and 3000 bp bands are three times brighter than the other bands in the ladder.

The recommended amount of size mar-ker to load on an agarose gel is 0.5-1 µg per lane (5-10 µl).

To demonstrate the mobility of the DNA fragments, 1 µg of marker was loaded onto a 1% agarose gel.

Concentration: 1 µg/10 µl

Pack size*: 100 µg in 1 ml (200 rxns) in loading buffer (0.25% bromphenol blue, 0.25% xylene cyanol FF, 40% sucrose in water).

 

Protein molecular size markers

Biotinylated Protein molecular weight markers

(MW 6500 – 205000)

UP344440

20 lanes

  • Determination of molecular weight of proteins by SDS-PAGE blotting with chimioluminescence revelation.

  • Two blue stained proteins visualize the transfer efficiency !

  • Ready to use : add 5-10 µl/well
Includes:

Reagent 1: biotinylated proteins (200 µl)

Reagent 2: Peroxidase conjugated Streptavidine (200µl)

Quantity sufficient for 20 western Blots detections

Reagent 1 is a ready-to-use mixture of defined proteins. 2 additionnal proteins are included to allow a vizualisation of the transfer of proteins from the gel to the blot. It is loaded on to gel (5-10 µl per well in 16cm2 gels and 5 µl per well in mini gels) for electrophoresis separation, then transferred to nitrocellulose sheets.

Reagent 2 is added to the secondary antibody during the immuno-detection steps. After final washes, the blot is detected by chimioluminescence (UptiLight UP99619A).

Technical Sheet

 

KDa

a2-Macroglobuline (Human Plasma)

205.0

b-Galactosidase (E. Coli)

116.0

Phosphorylase B (Rabbit Muscle)

97.4

Catalase (Bovine Lung)

58.1

Alcool Deshydrogenase (Horse liver)

39.8

Anhydrase carbonique (Bovine Erythrocytes)

29.0

Trypsin Inhibitor (Soja)

20.1

Lysozyme (Yolk egg)

14.3

Aprotinin (Bovine)

6.5

Blue colored dyes

ca 34 kda

ca 76 kda

Molecular weight of 2 additionnals blue colored dyes included : ca. 34 kD and 76 kDA

 

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