Cell Biology Tools

cell culture reagents


Buffers and Additives for cell biology

 

Buffers are choosen depending on their buffering features, notably their pKa should be in the range of working pH. More usefull buffers are HEPES, MOPS, Borate, Citrate…

 

AEBSF

UP401070

100mg

 

 

Borate

UP07044

UP401071

1g

Citrate

UP16878

UP401072 3x1g

MOPS

UP06200

UP401073 5x1g

HEPES

UP06194

Non toxic and more potent serine protease inhibitor more soluble and stable, than PMSF and DFP

4-(2-aminoethyl)benzeneSulfonyl Fluoride

MW 239.7

Technical Sheet - Security Sheet

PBS, sterile

UP30715

See the technical information for each buffering compound

This protease inhibitor is more useful for cell biology applications than others inhibitors for following reasons :

-

PMSF

DSP

AEBSF

Solubility (in water)

0.12 (a)

15.4

200

Stability

Low (b)

Mediocrous

Excellent

Inhibitor activity

-

-

-

trypsin/chymotrypsin

Good

Excellent

Good

plasmatic enzymes

Good or less

Good or less

Excellent

Toxicity (relative)

300

14

1

(a) Decreases at high ionic strength

(b) Need frequent additions

See also Proteases Inhibotors

Sucrose

UP25203

 

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